2023-01-13 Basic biology experiment “DNA amplification ~ PCR method ~”

"PCR" was a word that was not very familiar to the general public, but with the spread of the new coronavirus infection, everyone has heard of it. "PCR" is an abbreviation for polymerase chain reaction.
This technology can efficiently increase the number of copies of a targeted genomic region, making it possible to measure even a small sample.

The first to fourth photos show the reagents being mixed.
One that uses a pipette to accurately measure out and mix together primers for replicating DNA (DNA designed to attach to both ends of the region you want to increase) and enzymes.

When measuring a very small amount of reagent, it is important to see it carefully with your own eyes.

The fifth photo is a machine called a thermal cycler that manages temperature cycles.
If you set it according to the time and temperature you want the sample to react with, it will manage the temperature appropriately.

Once the PCR reaction is complete, mix the reagent with the staining solution and put it into the analyzer.
The 6th photo shows me measuring reagents to put them into the analyzer, and the 7th photo shows the analyzer (electrophoresis device).

In this class, Professor Tsukagoshi explained important points when using the tools, and Wang was able to understand the specific methods of conducting experiments.
By actually experimenting with your hands, you can learn the basic techniques necessary for conducting research in biotechnology-related fields.

There are Department of Biotechnology and Life Science of Department of Biotechnology and Life Science at many universities, but the first one to be established in Japan was at TUAT and Technology!

Department of Biotechnology and Life Science offers practical engineering education through a unique curriculum based on biochemistry, molecular biology, and medical engineering, enabling students to learn biotechnology efficiently and systematically.

Bioengineering is a field of research that seeks to understand the mechanisms of biological phenomena and apply them to make them useful in daily life.
In order to understand and utilize complex and advanced life phenomena at the molecular level, we use engineering techniques to create analysis techniques and research methods that are tailored to the various layers of living things, such as genes, proteins, cells, and individuals. One that approaches the mysteries of life!
Then, we will create new industries based on the knowledge gained from research on a wide range of living organisms.

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